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Maintain a moderate shaking speed during the washing process to avoid excessive speed, which may result in the shedding of proteins or antibodies from the membrane, and to ensure that the washing solution can fully contact every corner of the membrane

(2) Whole protein peptides: These peptides are derived from full-length self-proteins and can induce a broader immune response against multiple epitopes of the protein

doi: 10.1016/j.clinthera.2018.12.016 556 GoldenbergDLSimmsRWGeigerAKomaroffAL

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