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To analyze postprandial incretin secretion, we fasted PAR2-mutant and WT mice overnight followed by refeeding of normal chow diet (NCD) for 1 hour, a reliable time point as determined by a pilot experiment in WT mice on both C57BL/6N and C57BL/6J backgrounds (Supplemental Figure 1B)

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COQ10, additional Vitamin C and IM injections of Vitamin D are also available.

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