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Although doses of DPP-4 inhibitors that reduce enzyme activity in the intestinal lumen without prominent systemic effects (gut-selective) improve glucose tolerance and increase both GLP-1 and GIP, they are not as potent as doses that decrease plasma DPP-4 activity as well [19, 20]
Kanhai KMS, Zuiker R, Stavrakaki I, Gladdines W, Gaillard PJ, Klaassen ES, et al
Diabetes Obes Metab (2021) 23(2):50819
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Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining for apoptosis Apoptosis was also determined by the TUNEL assay using an in situ cell death detection kit conjugated with horse-radish peroxidase (POD) (Roche Applied Science, Indianapolis, IN, USA), according to the manufacturer's instructions
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