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Draw up medication Remove the seal on the top of the vial and wipe with alcohol Remove the cap off the needle Hold the syringe like you hold a pen or a dart, inserting the needle into the middle of the rubber stopper perpendicular, so the needle does not bend

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Jennis M, Kung CP, Basu S, Budina-Kolomets A, Leu JI, Khaku S

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Vota DM, Crisp RL, Nesse AB, Vittori DC

In whole-cell current clamp experiments, hyperpolarizing or depolarizing current pulses (500 ms, from 10 to 30 pA in 5-pA steps) were injected into neurons to determine input resistances or trigger action potentials