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The experiments revealed clear age-related declines as well as improvements after treatment: Aging neurons had lower GTP levels , including less free GTP near mitochondria

doi: 10.3390/microorganisms8060830 106 VaqueroC.LoiraI.HerasJ

Such protein-based biologics can be genetically fused to either the N-terminal or C-terminal end of albumin, or to both 6,7,8,9,10,11,12,13,14,15

Cells were cultured at 37 C in 5% CO 2 in Dulbeccos modified Eagles medium (DMEM) with high glucose (4.5 g/L) (DM-22, Omega, Tarzana, CA) supplemented with 10% dialysed fetal bovine serum (FBS) (FB-07, Omega, Tarzana, CA), 100 units/mL of penicillin, 100 g/mL of streptomycin (Gemini Bio-Products, Sacramento, CA), and either 0.5 or 2.0 mM L-glutamine (GS-60, Omega, Tarzana, CA)