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Mohamed, M.F., Brezden, A., Mohammad, H., Chmielewski, J., and Seleem, M.N., Biochem

Less necrosis developed in treated wounds, suggesting the peptide helped preserve marginally viable tissue that might otherwise have died

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the cells were then fixed in a 4% PFA solution for 15 min at room temperature and permeabilized right after in 0.25% Triton X-100 in PBS during 20 min at room temperature