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Mass Spectrometry confirms the molecule's identity

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The thermal cycling conditions consisted of the following: cDNA synthesis for 10 minutes at 50 C, initial denaturation and Taq Polymerase activation for 5 minutes at 95 C, followed by 40 cycles of denaturation at 95 C for 10 seconds and annealing/extension at 60 C for 30 seconds

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