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Pellet was resuspended in glucose-free RPMI medium supplemented with 2-NBDG (Thermo Fisher N13195) at 50 M and incubated for 30 min at 37 C

In in vitro models, ferroptosis inhibitors such as Lip-1 and DFO have demonstrated protective effects on iodinated contrast agent-stimulated HK-2 cells

Based upon the fact that the suppressed autophagy is usually associated with enhanced ER stress (Zhang et al

Step 5: Prioritize hydration

Such a setup would precisely delineate the NSC-derived paracrine factors (e.g., BDNF, VEGF [18, 32], TGF-) responsible for suppressing microglial activation and promoting an anti-inflammatory phenotype, independently of in vivo complexities

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