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We prepared custom diets: 2.5% Si-based agent containing AIN93M, and only AIN93M as the control (Oriental Yeast Co., Ltd., Tokyo, Japan)

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As different classes of drugs target different aspects of T2DM via distinct mechanisms of action, a combination of treatments is generally more effective at maintaining glycaemic control than monotherapy over the long term [12]

Briefly, RAW 264.7 cells were washed three times with phosphate buffer saline (PBS, Corning) and then incubated in ice-cold hypotonic lysing buffer (50 mM TrisHCl, 10 mM magnesium sulfate at pH 7.5) for 4 h at 4 C

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