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After SDSPAGE, the following antibodies were used: CDK11 (rabbit antiserum, 1:3000), P-T235-SF3B1 (rabbit antiserum, 1:3000), GST (Santa Cruz, sc-138, 1:200), SNIP1 (Proteintech, 14950-1-AP)
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However, during the chronic inflammation, the glial activation causes an increase of ROS production and other neurotoxic mediators, leading a neuronal damage [52]
In that situation, repeating the same treatment protocol may fail because peripheral nerve irritation, scar tissue, TMJ or muscle-related triggers, central sensitization, or another pain generator has not been identified
Beyond stability, it minimizes waste
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