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Iron overload also significantly increased the protein and gene expression of FTH/L and SLC40A1 in the iron metabolic pathway, which was rescued by theaflavins, indicating theaflavins' ability to inhibit ferroptosis by restoring iron metabolism (Fig

Including only males, while not ideal, allowed us to eliminate the effect of an important covariate (i.e

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After washing once with Annexin-V binding buffer, cells were acquired via flow cytometry (LSRII, BD Bioscience, Heidelberg, Germany) and data were analyzed with FlowJo X (FlowJo LLC, Ashland, OR, USA)