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The rules on peptides are in flux, and they might soon change to the point that DSIP, and other peptides on the restricted list, becomes more readily available

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After alkylation, 900 L of 100% ACN was added to the bead slurry to re-adsorb proteins onto the beads and to precipitate protein, and the beads were captured magnetically

The copy numbers of integrated genes were verified using real-time PCR (primers GshF-RT1/GshF-RT2, Additional file 1: Table S7) and calculated by a comparative C T method [27]

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