PC cells (1-5 10 6 /ml) were incubated with a probe at 37C for 20 min in a 35 mm porous glass bottom dish (Cellvis, USA) and the nuclei were stained using Hoechst (Beyotime)
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Cell death induced by the lack of Hh signalling is JNK independent Previous studies have shown that JNK signalling plays a vital role in cell morphology, cell invasion, and apoptosis 26, 31, 63, 64
Potential offlabel or alternative uses discussed in medical settings can include appetite control during weight regain risk periods, or metabolic support in people with insulin resistance, but these uses depend on local regulations and individual medical assessment
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