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The p70S6K1, p70S6K2, and GAPDH mRNA levels were quantified by qPCR using primers purchased from Qiagen

22 and 23)

This nonapeptide, first isolated from the cerebral venous blood of rabbits during sleep, has been shown to induce slow-wave sleep, modulate pain perception, and exhibit potential antioxidant and immunomodulatory properties

Determine Concentration (example: 10 mg vial 1 mL water = 10 mg/mL): Concentration (mg/mL) = Total Peptide (mg)Total BAC Water (mL) Calculate Dosage Volume (example: 1 mg dose 10 mg/mL = 0.1 mL): Volume to Draw (mL) = Desired Dose (mg)Concentration (mg/mL) Convert to Syringe Units: Using a standard U-100 (1 mL/100 unit) insulin syringe, 0.1 mL equals 10 units

Hursting SD, Switzer BR, French JE, Kari FW

Abstract Toxic protein aggregates can spread among neurons to promote human neurodegenerative disease pathology