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(88) in an 8-week randomized double-blind, placebo-controlled trial with 80 individuals experiencing episodic migraines, discovered that the combination of omega-3 PUFAs and nano-curcumin significantly reduced migraine attack frequency and serum levels of IL-1 in a synergistic manner, though the initial significant reduction in IL-1 gene expression did not remain significant after Bonferroni corrections
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For staining, 0.5 cm-long intestine pieces or mesenteric tissues were washed with PBS, permeabilized with 0.5% Triton X-100 and blocked with 5% donkey serum overnight
