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The copper complex is slightly more light-sensitive than most peptides store the reconstituted vial in the darker corner of your fridge or wrap it in foil
A Spectra Multicolor Broad Range Protein Ladder (ThermoFisher) was used as standard, and the gels were stained with Comassie Brilliant Blue (BioRad)
Feb 3 2022;14(3)doi:10.3390/nu14030652
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It also suppresses p38 MAPK signaling, another pathway involved in cellular stress responses
Compared with Control cells, p53 KO cells exhibited far fewer nutlin-3induced changes in gene expression, consistent with most changes observed in Control cells being p53-dependent (Fig 3A)
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