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The results obtained with the ELISA kits for mature BDNF [#DBD00 (R&D Systems), #EH0043 (FineTest) and #SK00752-01 (Aviscera Bioscience)] indicate that the antibodies used do not exhibit absolute specificity for this isoform

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Following fixation in 4% paraformaldehyde (PFA), 5 dpf Brn3c:mGFP larvae, which express membrane-bound GFP in hair cells, were rinsed twice with phosphate-buffered saline (PBS) for 10 min each and then once with dH 2 o for 20 min

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Instead of using one peptide in isolation, stacking pairs or groups of peptides in a way that supports multiple pathways simultaneously amplifying benefits without increasing dosage unnecessarily

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