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doi:10.1093/ibd/izy320 80Marehbian J, Arrighi HM, Hass S, et al

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The benzyl alcohol inhibits bacterial growth, which means that a reconstituted vial can, in principle, be used across multiple doses over a period of days to weeks rather than being discarded after a single use as would be required with plain sterile water

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If treating yourself, use a disposable needle and syringe only and discard responsibly after use

For beginners, start with 150 mcg daily for the first week to assess tolerance