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PAE is performed as an outpatient procedure, meaning that most patients can go home the same day

2006 PMID 16583442)

This DHFR-independent pathway replenishes the intracellular THF pool, sustaining 1 C metabolism for nucleotide synthesis and methylation reactions, thereby maintaining cancer cell proliferation and contributing to acquired resistance to DHFR-targeted antifolate therapies [157, 158]

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