To evaluate the mitochondrial membrane potential by flow cytometry, the cells were therefore detached by using trypLE as described in 2.1, re-suspended the cells in complete cell culture medium (1 10 6 /ml) and treated with rotenone (0.5 M, 2 h) in an eppendorph tube
As fat cells accumulate, this can create a worsened, uneven surface
PMCID: PMC3662563 Greeley CS, Donaruma-Kwoh M, Vettimattam M, Lobo C, Williard C, Mazur L
The injection is given into a muscle (known as an intramuscular injection), because of this you may experience some tenderness, swelling or itching in the area where your injection was given
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Physiology 121 (1), 1238