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The levels of mRNA of target genes were normalized to GAPDH using a QuantStudio 7 Real-Time PCR system (Thermo Fisher, MA, USA)

doi: 10.1038/nrd.2017.162 33 KhomichO

DSIP (Delta Sleep-Inducing Peptide) is a naturally occurring nonapeptide first isolated in 1977 that helps regulate deep, restorative sleep without the sedative hangover of traditional sleep medications

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There are limitations to the current study