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Very short needles (4mm) may not fully penetrate the dermis in areas with exceptionally thick skin

These concentrations allow for practical injection volumes at each dose level in the titration schedule

Then, the animals proceeded to a PR schedule of reinforcement where they were tested across 4 sessions at variable doses of fentanyl (0-10 g/kg/inf, i.v.) in a randomized order while receiving a stable dose of semaglutide (0.1 mg/kg, s.c.) or vehicle (0.9% saline, s.c.)