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The mRNA levels for modulated genes were determined by reverse transcription of total RNA followed by quantitative real-time PCR analysis (qRT-PCR) on a Quant Studio 6 Flex system (Applied Biosystems, Life Technologies, U.S.) using SYBR PCR Master Mix reagent kits (Takara, Japan) following the manufacturers protocol

The peptides possible role in mammalian cell cultures suggests its potential relevance in optimizing recombinant protein production and biopharmaceutical research

These findings epitomize the contradictory nature of lipid peroxidation in cancer processes

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Some studies also suggest that l carnitine use may boost antioxidant capacity