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2) Resuspend the cells in 10 mL of Equilibration buffer (LE buffer) with appropriate amount of PMSF (Final working concentration can be 1mM) and add lysozyme (Final working concentration is 0.2-0.4 mg/mL), or other protease inhibitors cocktail added

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the SAG thus assimilated by the tissues is hydrolysed by cytoplasmic thioesterase and, by hydrolysis of the acetyl group, produces reduced GSH which is available for all the biological functions wherein it is required

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