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Western Blot Total proteins were extracted in RIPA buffer (Sigma-Aldrich) that was supplemented with protease inhibitor cocktail (100 Sigma-Aldrich)

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Benefits for men: Restores natural testosterone production Prevents testicular atrophy during TRT or steroid use Maintains fertility Can help recover natural production after suppression Dosing: 100-200mcg subcutaneous, 2-3x daily (pulsatile dosing important) Often used in conjunction with TRT to prevent shutdown Best for: Men who want to preserve testicular function while on TRT

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