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BMDMs were cultured in 1640 medium with 10% FBS, 100 U/mL penicillin, 100 g/mL streptomycin, and 20 ng/mL M-CSF (prime gene, China) for one week

Both require identical storagelyophilized at -20C, reconstituted at 2-8C, used within 28 days

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12 Replacement of GLP-1 His 7 with Phe 7 does not influence either its receptor affinity or cAMP activity, indicating that the aromaticity of His is crucial to its activity