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We confirmed activation of this conical adrenergic-driven pathway with 10 nM isoproterenol stimulation and were able to inhibit isoproterenol-induced pSer 16 with carvedilol (1, 2, and 1 adrenergic receptor inhibitor) 41 preincubation

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2.3 Biochemical assays Blood samples obtained as described in Section 2.2 were centrifuged (3,000 rpm, 15 min, 4 C), and the serum was stored at 80 C until analysis

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