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After 24 hpi, each well was washed, fixed with 4% Histofix (Roth), mounted with ProLong Gold antifade mountant (Thermo Fisher Scientific), and visualized at 64 magnification with an Axio Observer microscope (Carl Zeiss Microscopy) using bright field and fluorescence channels (596/621 nm)

Toxicity of glutathione-binding metals: a review of targets and mechanisms

doi: 10.1016/j.celrep.2022.111314, 103 NiedermanR.WesternoffT.LeeC.MarkL

These findings may provide a safe and effective strategy to enhance ferroptosis-driven antitumor immunity, and the completion of clinical trials is needed to draw conclusion about the side effect

One of the key pathways through which MOTS-c exerts its effects is by activating AMP-activated protein kinase (AMPK) signaling

It contains around 40% ABV (80 proof)