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Fluorescence was measured after setting up the reaction in the kinetic mode for 60 minutes at 37 C by TECAN kinetic analysis (excitation 360 nM, emission 460 nM, 30-second reading interval) on a Safire II microplate reader (TECAN)

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Verify each dose with units = mg 2 (e.g., 100 mg = 50 units, 200 mg = 100 units) [3] [4]