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snRNA-seq and analysis For isolation of nuclei from gWAT, fresh adipose tissue was minced in nucleus preparation buffer (10 mM HEPES, 1.5 mM MgCl 2 , 10 mM KCl, 250 mM sucrose, 0.001% NP-40) with 0.2 U l Roche Protector RNase inhibitor followed by dounce homogenization

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Each ml of sterile Aqueous solution contains