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After blocking with 2% bovine serum albumin (BSA) in PBS for 1 h at room temperature, cells were incubated overnight at 4 C with primary antibodies (diluted 1:100 in 1% BSA)

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NAD+ (Nicotinamide Adenine Dinucleotide) is an essential part of the bodys cellular function and is present in every single cell in the body