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Beyond genetics, obesity itself reduces GLP-1 secretion in a harmful cycle: excess weight dampens L-cell function, lowering hormone production, which allows more overeating, which perpetuates weight gain

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The PCR reactions were carried out in 20-l reaction volumes using the SYBR Green PCR Master Mix (Applied Biosystems, USA) with 600 nM oligonucleotide primers and cDNA reverse-transcribed from 10 ng or 40 ng total RNA

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Pyruvate anaplerosis is a mechanism of resistance to Pharmacological glutaminase Inhibition in triple-receptor negative breast cancer