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These cross-species findings reinforce the mechanistic plausibility of our results and highlight Spirulina platensis as a robust nutraceutical candidate capable of modulating oxidative stress, apoptosis, and lipid metabolism in diverse biological systems
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Its 3-letter sequence, Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Ser-Arg-Asn-His (31 residues as tabulated with N-terminal modification), features a single methionine that analysts should note as a potential oxidation site during method development, alongside multiple basic residues (Arg, Lys, His) that dominate its charge state envelope in positive-mode ESI-MS
Cells were harvested by centrifugation (5500 g ) and flash frozen with liquid N 2
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