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Whole cell and mitochondrial proteomics After the indicated culture conditions, cell pellets were processed as per immunoblot protocol and lysed with lysis buffer (50 mM Tris-Cl pH 7.5, 150 mM NaCl, 1 mM EDTA, 2% Triton X-100) supplemented with protease inhibitors and phosphatase inhibitors (Roche)

We prioritize data reliability, controlled conditions, and the advancement of best practices in peptide reconstitution and compound stability, supporting precise and reproducible research outcomes

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doi: 10.1016/j.neuropharm.2017.08.010 66 EsslingerCSCybulskiKARhoderickJF

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doi:10.1016/j.ajoc.2021.101171