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For test subjects on a 2,000 g/week dosing schedule, this would equate to five 400 g injections per week or twice-daily 200 g doses

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To evaluate intestinal barrier integrity, rectal sections underwent immunofluorescence staining for ZO-1 using a rabbit primary antibody and an Alexa Fluor 488 secondary antibody (green), and the nuclei were counterstained with DAPI (blue)

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Metabolic slowing and reduced oxidative damage with sustained caloric restriction support the rate of living and oxidative damage theories of aging

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