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After the washing step, the TMB substrate solution was added to each well followed by incubation at 37 C for 20 min

The Intracellular ferrous iron can be oxidized and exported by the membrane protein ferroportin (FPN), which currently is the unique iron efflux pump discovered that can release iron from cells ( The intracellular labile iron levels could be enhanced via TF-mediated iron absorption or selective autophagy of ferritin

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