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Briefly, INS-1 832/3 SNAP/FLAG-hGLP-1R cells were seeded on gridded coverslips (MatTek) and transfected with EGFP-VAPB before labelling with MitoTracker Red CMXRos and SNAP-Surface Alexa Fluor 647 and stimulation with 100 nM exendin-4 for 5 minutes

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[PMC free article: PMC18403] [PubMed: 10518567] 309

Dysregulation of the HPA axis contributes to suppression of transcription of the brain-derived neurotrophic factor (BDNF) gene, thereby reducing the synthesis and secretion of BDNF protein, a nerve growth factor family (252)

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