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To perceive particular aspects of the injured hind limb contracture presentation, measurements were made at all 3 times to all rats while rats were awake, anesthetized, or immediately after sacrifice

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doi: 10.3389/fimmu.2019.01027 158 KinseySGNaiduPSCravattBFDudleyDTLichtmanAH

Briefly, rat striatal tissues were homogenized in ice-cold lysis buffer (50 mM Tris, 150 mM NaCl, 2 mM EDTA, 1% NP-40, 0.5% sodium deoxycholate, pH = 7.4, with protease inhibitor (Sigma-Aldrich)), and rotated for 1 h at 4 C

Avoid temperature fluctuations and keep vials tightly sealed