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After 48 h, the cells were lysed, and the firefly and Renilla luciferase activities were measured with the Dual-Luciferase Reporter Assay System (Promega)

This threshold was chosen to prioritize pathways with at least moderate evidence of a link to AD, ensuring that our network was focused on biologically relevant pathways while still being inclusive enough to capture a broad range of potential mechanisms

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Hyperthermia, cerebral edema, and outcome in intracerebral hemorrhage: too darn hot

Data were re-analysed according to the per-protocol definitions and found only minor changes to AUC 0 and C max , with no effect on the conclusions