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Sterile water can be used but shortens reconstituted peptide shelf life to 72 hours versus 28 days with bacteriostatic water

Zou Y, Sun X, Wang Y, Yan C, Liu Y, Li J, et al

The cells were treated with RIPA containing 5% protease and phosphatase inhibitor, the suspended cells were lysed with sonication (14 3 s with 5 s breaks, 12% power) on ice

Medical writing assistance, undertaken at the direction of the authors, comprised literature searches, drafting of the manuscript, editing of the manuscript, preparation of figures and tables, and contributions to the interpretation of the literature in the discussion

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148, no