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This enables the use of low dose aspirin as a selective antiplatelet drug (Figure 2) At higher doses, aspirin inhibits both isoforms of COX (Figure 2)

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The resulting protein was centrifuged to remove any aggregates and loaded onto a Superdex 200 16/600 size exclusion chromatography (SEC) column equilibrated in SEC buffer for crystallisation (10 mM Tris-HCl pH 8.0, 200 mM KCl, 0.5 mM PMSF, 1 mM TCEP) 31 or NMR (50 mM mono/dibasic sodium phosphate buffer pH 8.0, 150 mM NaCl, 1 mM TCEP)

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