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These variants have varied levels of function, ranging from partial to complete loss of function

Optional: ATP buffer: Tris HCl, pH 7.4, 50 mM, ATP 2 mM, MgSO4 10 mM Elution buffer: Tris base, pH 7.4, 125 mM, NaCl 150 mM, Triton X-100 0.1 % (v/v), Reduced glutathione 50 mM, DTT 1 mM Note: Optimal buffer conditions may vary depending on the protein of interest

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