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Volume: 16.91 fl oz
Briefly, the lyophilized internal standards were reconstituted according to the manufacturers specifications, and the daily working solution was prepared by diluting the reconstituted internal standards 1:100 (v/v) using the extraction solution
Measurements were validated using the Wave software (Agilent, Santa Clara, California, USA) and normalized to DNA concentrations per well, analyzed in stained cells (Hoechst 33342) using a plate reader (Tecan Infinite M200, Tecan Group Ltd., Mnnedorf, Switzerland) with an excitation wavelength of 350 nm and an emission wavelength of 461 nm
4.2 Peptides suppressing the activity of transcription factors Interference peptides (iPeps), mutational transcription factor versions, are inert and able to inhibit the transcriptional program by sequestering their binding partners tight (Sorolla et al., 2020)
