Three discs were frozen and ground, and 80 l of 2x SDS loading buffer (125 mM Tris-HCl, pH 6.8, 2% SDS, 0.05 Bromophenol blue, 20% glycerol, 200 mM -Mercaptoethanol) was added
10.1016/j.molbiopara.2014.03.003 Mol
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