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Add 120 mM DTT (or other reducing agent) to the buffer

Arginine synthesis is impaired when small intestine and kidney function is impaired, thus creating a dietary requirement for arginine

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Kolwicz SC Jr, Olson DP, Marney LC, Garcia-Menendez L, Synovec RE, Tian R (2012) Cardiac-specific deletion of acetyl CoA carboxylase 2 prevents metabolic remodeling during pressure-overload hypertrophy