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The genes analyzed included insulin receptor (InsR), insulin receptor substrate-1 (IRS-1), tumor necrosis factor-alpha (TNF-), nuclear factor-kappa B-1 (NFB-1), interleukin-6 (IL-6), caspase-3, Bcl-2-associated protein X (Bax), and B-cell lymphoma 2 (Bcl-2), with -actin selected as the endogenous housekeeping gene (Table 6) 67

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