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In research settings, interest may include metabolic signalling, glucose response models, appetite-related pathways, energy balance research, receptor comparison and cardiometabolic research frameworks

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The sample size was selected in accordance with previous studies using comparable models and endpoints, which reported adequate statistical power to detect treatment-related differences while minimizing animal use in compliance with the 3Rs principle 32

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Emerging nano-formulations using mesoporous silica nanoparticles or nanoclay composites can deliver PGPR directly to root surfaces with unprecedented precision, though cost currently limits field-scale adoption

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