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The gap between effective storage and complete waste comes down to three variables most SOPs never mention: the temperature stability window, the freeze-thaw tolerance limit, and the bacterial contamination timeline after first puncture

Pevec D, Novinscak T, Brcic L, Sipos K, Jukic I, Staresinic M, et al

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Gharibo says that it depends on the patient and the clinicians judgment as far as which injection should be performed, and which steroid should be injected, and whether a cortisone or steroid should be injected at all. For example, nerve blocks for facet joints and trigger point injections may not involve steroid injections

These bacteria are beneficial in the large intestine