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2020 doi: 10.1007/s10571-020-00796-4

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On the day of the assay, serial dilutions (7-fold dilutions, 7 concentrations per compound and one well containing only assay buffer) of test compounds were performed in duplicate in assay buffer to appropriate concentrations in 96-well plates (Greiner, U-shape #GR-650201) using a Biomek i7 liquid handler

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-Glutamylcyclotransferase knockdown inhibits growth of lung cancer cells through G0/G1 phase arrest