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Melatonin, through the MT1 receptor, inhibits NF-, modulating its translocation to the nucleus and binding to DNA, which leads to the negative regulation of COX-2 [28,30]

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The eluted fractions were analyzed by SDSPAGE, and protein bands were visualized using a ChemiDoc imaging system (Bio-Rad)

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Robust randomized trials and meta-analyses consistently show significant reductions in major adverse cardiovascular events, meaningful weight loss, improved blood pressure control, and favorable renal outcomes, including slower decline in estimated glomerular filtration rate and reduced albuminuria